Application
Antibodies tested include p-FRAP (Ser 2448): (A,B,C) and FRAP (30): (D,E,F).Application
Antibodies tested include p-FRAP (Ser 2448): (A,B) and FRAP (30): (C,D).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-mTOR Antibody (Ser 2448) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 2448 of mTOR of human origin |
| Modification | Phosphoralated |
| Modification Notes | The PIK-related kinases include Atm, DNA-PKCS and FRAP. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration and the appearance of dilated blood vessels in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding enzyme that is composed of a large subunit, DNA-PKCS, and two smaller subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind to rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has been implicated in the regulation of p27 and p21 expression. FRAP autophosphorylates at Ser2481 under translationally repressive conditions. Phosphorylation of FRAP at Ser2448 is mediated by p70S6 kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 2448 phosphorylated mTOR of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 2448 of mTOR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 2448 of mTOR of human origin |
| Entrez Gene | 2475 |
|---|
Application
Antibodies tested include p-FRAP (Ser 2448): (A,B,C) and FRAP (30): (D,E,F).
Application
Antibodies tested include p-FRAP (Ser 2448): (A,B) and FRAP (30): (C,D).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 2448 phosphorylated mTOR of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
