Application
Western blot analysis of MEF-2 expression in non-transfected: (A) and human MEF-2 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of MEF-2 expression in non-transfected 293T: (A), mouse MEF-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of MEF-2C expression in non-transfected 293T: (A), mouse MEF-2C transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of MEF-2 expression in non-transfected 293T: (A), human MEF-2 transfected 293T: (B) and Daudi (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain tissue showing nuclear localization.Application
Western blot analysis of MEF-2 expression in BC3H1 (A) whole cell lysate and rat brain tissue extract (B).| Product Name | MEF-2 Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of MEF-2 of human origin |
| Modification Notes | The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C, and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of MEF-2A and, to a lesser extent, MEF-2C and MEF-2D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of MEF-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of MEF-2 of human origin |
| Entrez Gene | 4205 |
|---|
Application
Western blot analysis of MEF-2 expression in non-transfected: (A) and human MEF-2 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of MEF-2 expression in non-transfected 293T: (A), mouse MEF-2 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of MEF-2C expression in non-transfected 293T: (A), mouse MEF-2C transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of MEF-2 expression in non-transfected 293T: (A), human MEF-2 transfected 293T: (B) and Daudi (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse brain tissue showing nuclear localization.
Application
Western blot analysis of MEF-2 expression in BC3H1 (A) whole cell lysate and rat brain tissue extract (B).| Application Notes | recommended for detection of MEF-2A and, to a lesser extent, MEF-2C and MEF-2D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
