Application
Western blot analysis of CENP-A expression in non-transfected: (A) and mouse CENP-A transfected: (B) 293T whole cell lysates.| Product Name | CENP-A Antibody (M-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CENP-A of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-85 mapping at the N-terminus of CENP-A of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-85 mapping at the N-terminus of CENP-A of mouse origin |
| Entrez Gene | 12615 |
|---|
Application
Western blot analysis of CENP-A expression in non-transfected: (A) and mouse CENP-A transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CENP-A of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
