Application
Western blot analysis of p57 expression in A-673 (A,B), Jurkat (C,D) and HeLa (E,F) whole cell lysates (A,C,E) and nuclear extracts (B,D,F).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human fetal kidney showing nuclear staining.| Product Name | p57 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of p57 of human origin |
| Modification Notes | Cell cycle progression is regulated by a series of cyclin-dependent kinases that consist of catalytic subunits designated Cdks and activating subunits designated cyclins. Orderly progression through the cell cycle requires the activation and inactivation of different cyclin-Cdks at appropriate times. A series of proteins has been described that function as mitotic inhibitors. These include p21, the levels of which are elevated upon DNA damage in G1 in a p53-dependent manner; p16; and p16-related inhibitors, designated p15, p18 and p19. A p21-related protein, p27, has been described as a negative regulator of G1 progression and has been speculated to function as a possible mediator of TGF b-induced G1 arrest. A member of the p21/p27 family of mitotic inhibitory proteins, p57 (also designated Kip2), is a potent, tight-binding cyclin-dependent inhibitor of several G1 cyclin/Cdk complexes. Overexpression of p57 arrests cells in G1. Unlike p21, p57 is not regulated by p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p57 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of p57 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of p57 of human origin |
| Entrez Gene | 1028 |
|---|
Application
Western blot analysis of p57 expression in A-673 (A,B), Jurkat (C,D) and HeLa (E,F) whole cell lysates (A,C,E) and nuclear extracts (B,D,F).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human fetal kidney showing nuclear staining.| Application Notes | recommended for detection of p57 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
