Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.Application
Control Input (A). Antibodies used included TRβ1 (J51): and TRβ1 (J52): (B), p300 (C-20): , p300 (H-272): and p300 (N-15): (C), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): , RelB (C-19): and p300 (N-15): . Data kindly provided by A. Hoffmann.Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.Application
Western blot analysis of p300 expression in non-transfected control (A) and p300 siRNA transfected (B) A-431 cells. Blot probed with p300 (N-15): . Lamin A/C (636): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.Application
Western blot analysis of p300 expression in A-431 nuclear extract.| Product Name | p300 Antibody (N-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of p300 of human origin |
| Modification Notes | Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of p300 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of p300 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of p300 of human origin |
| Entrez Gene | 2033 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells.
Application
Antibodies tested include CBP (A-22): , p300 (N-15): , PCAF (H-369): , and Brg-1 (H-88): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- thymocytes or Rag-/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press.
Application
Control Input (A). Antibodies used included TRβ1 (J51): and TRβ1 (J52): (B), p300 (C-20): , p300 (H-272): and p300 (N-15): (C), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
IP's permorned without (A) and with (B) primary antibody. Antibodies tested include NFκB p65 (A): , RelB (C-19): and p300 (N-15): . Data kindly provided by A. Hoffmann.
Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
Application
Western blot analysis of p300 expression in non-transfected control (A) and p300 siRNA transfected (B) A-431 cells. Blot probed with p300 (N-15): . Lamin A/C (636): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
Application
Western blot analysis of p300 expression in A-431 nuclear extract.| Application Notes | recommended for detection of p300 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
