Application
Western blot analysis of cyclin A expression in SK-BR-3 (A), HuT 78 (B) and U-937 (C) whole cell lysates.Application
Western blot analysis of cyclin A expression in K-562 (A) and HuT 78 (B) whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by APC-Cy7-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-Cy5-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-Cy5-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by FITC-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by APC-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of cyclin A expression in K-562 (A) and SK-BR-3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and nuclear staining.Application
Western blot analysis of cyclin A expression in K-562 (A) and 3611-RF (B) whole cell lysates.| Product Name | cyclin A Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of cyclin A of mouse origin |
| Modification Notes | The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of cyclin A of mouse, rat and human origin by WB, IP, KA, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of cyclin A of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of cyclin A of mouse origin |
| Entrez Gene | 890 |
|---|
Application
Western blot analysis of cyclin A expression in SK-BR-3 (A), HuT 78 (B) and U-937 (C) whole cell lysates.
Application
Western blot analysis of cyclin A expression in K-562 (A) and HuT 78 (B) whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by APC-Cy7-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-Cy5-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-Cy5-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by FITC-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by APC-conjugated goat anti-rabbit IgG F(ab')2: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with cyclin A (C-19), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of cyclin A expression in K-562 (A) and SK-BR-3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and nuclear staining.
Application
Western blot analysis of cyclin A expression in K-562 (A) and 3611-RF (B) whole cell lysates.| Application Notes | recommended for detection of cyclin A of mouse, rat and human origin by WB, IP, KA, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
