| Product Name | arfaptin 1 Antibody (H-69) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-69 mapping at the N-terminus of arfaptin 1 of human origin |
| Modification Notes | ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex (1). Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5-prime-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer (1). Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF (2). It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF (3). POR1 (also designated Arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia (3,4). POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner (5-7). The association of POR1 with ARF6 stimulates induction of actin polymerization (3). POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure (6,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of arfaptin 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-69 mapping at the N-terminus of arfaptin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-69 mapping at the N-terminus of arfaptin 1 of human origin |
| Entrez Gene | 27236 |
|---|
| Application Notes | recommended for detection of arfaptin 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
