Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of girdin expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.| Product Name | girdin Antibody (T-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Girdin, also known as CCDC88A (coiled-coil domain-containing protein 88A), APE, GRDN, GIV or HkRP1, is a 1,871 amino acid protein that, via interactions with phosphoinositides, localizes to the cell membrane and to the cytosol. Expressed ubiquitously, girdin functions to enhance the activity of PI 3-kinase-dependent phosphorylation of proteins, such as Akt1, thereby inducing the phosphorylation of downstream protein targets and, ultimately, regulating DNA replication and cellular proliferation. Additionally, girdin is crucial for cell migration and is responsible for both maintaining the structural integrity of the Actin cytoskeleton and for regulating the formation of Actin stress fibers. Girdin exists as a homodimer that can itself be phosphorylated—an event that delocalizes girdin from the cell membrane, thus allowing it to participate in cell migration events. Two isoforms of girdin exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of girdin isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC)P_ and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of girdin of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of girdin of human origin |
| Entrez Gene | 55704 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of girdin expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of girdin isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC)P_ and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
