Application
Western blot analysis of phosphorylated Smad2 expression in untreated (A) and IL-4-treated (B) HeLa cell extracts.| Product Name | p-Smad2 Antibody (Thr 220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad proteins. Mouse, rat and human Smad2 are subject to phosphorylation by TGF∫ receptors, and Thr 220 is one of the sites that is targeted for phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Thr 220 phosphorylated Smad2 of mouse rat and human origin by WB and IP |
|---|---|
| Tissue Specificity | recommended for detection of Thr 220 phosphorylated Smad2 of mouse, rat and human origin by WB and IP |
| Entrez Gene | 4087 |
|---|
Application
Western blot analysis of phosphorylated Smad2 expression in untreated (A) and IL-4-treated (B) HeLa cell extracts.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
