Application
Western blot analysis of APPBP1 expression in non-transfected (A) and human APPBP1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane localization.| Product Name | APPBP1 Antibody (D-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | APPBP1 (∫-Amyloid precursor protein-binding protein 1), also known as NAE1 (NEDD8-activating enzyme E1 regulatory subunit 1), HPP1 or ula-1, is a member of the ubiquitin-activating E1 family. In fetal tissues APPBP1 is widely expressed and in adult tissues it is expressed throughout the brain. APPBP1 is a cell membrane associated protein and functions as the regulatory subunit in a heterodimer with UBA3. The APPBP1/UBA3 complex binds to and activates NEDD8, a ubiquitin-like protein involved in signal transduction, cell proliferation and development. This suggests that APPBP1 affects a variety of cellular functions. In addition, APPBP1 is essential for cell cycle progression through the S/M checkpoint. More specifically, it inhibits the entry into S phase and promotes entry into M phase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of APPBP1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of APPBP1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 8883 |
|---|
Application
Western blot analysis of APPBP1 expression in non-transfected (A) and human APPBP1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
