Application
Western blot analysis of RFC-1 expression in NIH/3T3 whole cell lysate.| Product Name | RFC-1 Antibody (M-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Reduced folate carrier protein (RFC-1), also designated folate transporter 1, placental folate transporter (FOLT), or intestinal folate carrier (IFC-1), is a multi-pass membrane protein that acts as a transporter for folate intake. In human placental choriocarcinoma cells, folate intake occurs via potocytosis, a mechanism that couples three components: folate receptor, folate transporter and a H(+)-pump. RFC-1 is a heavily glycosylated protein that is primarily detected in liver and placenta. RFC-1 mediates the uptake of methotrexate (MTX), the antifolate drug widely used as both an anticancer chemotherapeutic drug and as an immunosuppressive agent. MTX mimics natural folates to inhibit critical cellular biosynthetic pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RFC-1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 239-300 mapping within an internal region of RFC-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 239-300 mapping within an internal region of RFC-1 of mouse origin |
| Entrez Gene | 6573 |
|---|
Application
Western blot analysis of RFC-1 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of RFC-1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
