Appliation
western blot analysis of Kaiso expression in non-transfected HCT 116 (A,C) and Kaiso transfected HCT 116 (B,D) whole cell lysates.| Product Name | Kaiso Antibody (H-154) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 345-498 mapping within an internal region of Kaiso of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The POZ-zinc finger protein Kaiso is a member of the BTB/POZ family of zinc finger transcription factors implicated in embryonic development and cancer. Kaiso, also known as ZNF-kaiso, maps to human chromosome Xq23 and encodes a 627 amino acid protein. Kaiso is a transcriptional repressor that contains an amino-terminal BTB-POZ protein-protein interaction domain and three carboxy-terminal zinc finger domains of the C2H2 DNA-binding type. The zinc-finger domains of Kaiso specifically recognize symmetrically methylated DNA sequences in vitro . Kaiso is known to associate with p120 catenin. Kaiso functions throughout development, and its repressor functions are most apparant in the context of neural tissues. Kaiso is predominantly localized to the nucleus and is expressed in the brain, eye, ear, branchial arches and spinal cord as well as NIH3T3 cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 345-498 mapping within an internal region of Kaiso of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 345-498 mapping within an internal region of Kaiso of human origin |
| Entrez Gene | 10009 |
|---|
Appliation
western blot analysis of Kaiso expression in non-transfected HCT 116 (A,C) and Kaiso transfected HCT 116 (B,D) whole cell lysates.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
