Application
Antibodies tested include p-Raf-1 (Ser 301): (A,B,C) and Raf-1 (540): (D,E,F).Application
Antibodies tested include p-Raf-1 (Ser 301): (A, B, C) and Raf-1 (540): (D, E, F).Application
Western blot analysis of Raf-1 phosphorylation in non-transfected: (A) and human Raf-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) NIH/3T3 whole cell lysates.Application
Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) UV-treated Jurkat whole cell lysates.| Product Name | p-Raf-1 Antibody (Ser 301) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338. Mouse, rat and human Raf-1 are phosphorylated, upon DNA damage, on Ser 301. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 301 phosphorylated Raf-1 of mouse rat human and Xenopus origin by WB and IP also reactive with additional species including bovine and canine |
|---|---|
| Tissue Specificity | recommended for detection of Ser 301 phosphorylated Raf-1 of mouse, rat, human and Xenopus origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 5894 |
|---|
Application
Antibodies tested include p-Raf-1 (Ser 301): (A,B,C) and Raf-1 (540): (D,E,F).
Application
Antibodies tested include p-Raf-1 (Ser 301): (A, B, C) and Raf-1 (540): (D, E, F).
Application
Western blot analysis of Raf-1 phosphorylation in non-transfected: (A) and human Raf-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) NIH/3T3 whole cell lysates.
Application
Western blot analysis of Raf-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) UV-treated Jurkat whole cell lysates.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
