Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Aprataxin expression in non-transfected: (A) and mouse Aprataxin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Aprataxin expression in HeLa nuclear extract.| Product Name | Aprataxin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Aprataxin is a nuclear protein, present in both the nucleoplasm and the nucleolus, which is a member of the histidine triad (HIT) superfamily. Aprataxin is involved in DNA single-strand break repair, mediating protein-protein interactions with molecules responding to DNA damage. Aprataxin contains three conserved domains: an N-terminal forkhead-associated (FHA) domain which mediates protein-protein interactions, a HIT domain that is similar to Hint, and a C-terminal zinc finger domain. Loss of function mutations in APTX, the gene encoding for Aprataxin, destabilize the Aprataxin protein and result in a rare neurological disorder known as ataxia-oculomotor apraxia, characterized by abnormal movements of the head and eyes. These mutations either target the HIT domain or truncate the protein N-terminal to a zinc finger. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Aprataxin isoforms 1-10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 57-356 mapping at the C-terminus of Aprataxin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 57-356 mapping at the C-terminus of Aprataxin of human origin |
| Entrez Gene | 54840 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Aprataxin expression in non-transfected: (A) and mouse Aprataxin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Aprataxin expression in HeLa nuclear extract.| Application Notes | recommended for detection of Aprataxin isoforms 1-10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
