Application
Western blot analysis of phosphorylated αPAK expression in untreated (A) and forskolin-treated (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-αPAK Antibody (Thr 212) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | p21-activated kinases (PAK) are serine/threonine kinases that link Rho GTPases to cytoskeletal reorganization and nuclear signaling. Three common isoforms are PAK1, PAK2, and PAK3 (also known as åPAK p68, ∫PAK p65 and ©PAK p62, respectively). å, ∫ and ©PAK isoforms associate with Rac 1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and mediating their autophosphorylation. ©PAK can undergo phosphorylation on Ser-19, Ser-141 and Thr-402, and phosphorylation of Ser-141 and Thr-402 correlates with ©PAK activation. Autophosphorylation of åPAK Thr-423 (Thr-402 for ∫PAK and Thr-421 for ©PAK) is catalyzed by Cdc42 and is required for kinase activation of PAK. Once phosphorylated and their affinity for Rac/Cdc42 reduced, PAK isoforms disassociate from the complex to seek downstream substrates. One such substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 212 phosphorylated &alphaPAK of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 212 of &alpha PAK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 212 of αPAK of human origin |
| Entrez Gene | 5058 |
|---|
Application
Western blot analysis of phosphorylated αPAK expression in untreated (A) and forskolin-treated (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Thr 212 phosphorylated &alphaPAK of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
