| Product Name | DOT1 Antibody (Y-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Yeast disruptor of telomeric silencing-1 (DOT1) functions in gene silencing at the pachytene checkpoint during the meiotic cell cycle. This owes to the fact that DOT1 methylates Histone H3 at lysine 79. Methylation of histone proteins affects chromatin structure to regulate gene expression, in this case, resulting in silencing of genes located near chromosome telomeres. This methylation limits silencing to discrete loci by preventing the binding of Sir proteins along the genome, and as these interacting proteins are conserved across species, similar mechanisms likely exist in other eukaryotes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DOT1 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 293-582 mapping at the C-terminus of DOT1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 293-582 mapping at the C-terminus of DOT1 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of DOT1 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
