Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondrial localization.Application
Western blot analysis of COX5b expression in Hep G2 whole cell lysate (A) and human heart tissue extract (B).| Product Name | COX5b Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Found in the inner mitochondrial membrane, COX5 is the heme A-containing chain of the oxidase family that converts one molecule of oxygen and four molecules of hydrogen to two molecules of water. Two isoforms of COX5 exist, COX5a and COX5b. When oxygen levels within the cell are high, transcription of COX5a (the aerobic isoform) is up-regulated as the rate of cellular respiration increases. Conversely, when oxygen levels are low, COX5b (the hypoxic isoform) transcription increases and functions to maximize the turnover rate of the COX apoenzyme. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of COX5b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-129 mapping at the C-terminus of COX5b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-129 mapping at the C-terminus of COX5b of human origin |
| Entrez Gene | 1329 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondrial localization.
Application
Western blot analysis of COX5b expression in Hep G2 whole cell lysate (A) and human heart tissue extract (B).| Application Notes | recommended for detection of COX5b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
