Application
Antibodies tested include p-Op18 (Ser 25): (A,B) and Op18 (A-4): (C,D).Application
Western blot analysis of Op18 phosphorylation in untreated (A), PMA treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lysatesApplication
Western blot analysis of Op18 (Ser 25) phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lystates.Application
Western blot analysis of phosphorylated Op18 expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-Op18 Antibody (Ser 25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Oncoprotein 18 (Op18) is a regulator of microtubule (MT) dynamics. Op18 is a target for both cell cycle and cell surface receptor-coupled kinase systems, and phosphorylation of Op18 on specific combinations of amino acid residues has been shown to switch off MT-destabilizing activity of Op18 (1,2). Induced expression of the catalytic subunit of cAMP-dependent protein kinase (PKA) results in a dramatic increase in cellular MT polymer content concomitant with phosphorylation and partial degradation of Op18 (2,3). Phosphorylation of Op18 on two specific sites, Serine 16 and Serine 63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells (1,3,4). PKA phos-phorylation downregulates the MT-destabilizing activity of Op18, which in turn promotes increased tubulin polymerization (1,5). Op18 has the potential to regulate the MT system in response to external signals such as cAMP-linked agonists (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 25 phosphorylated Op18 of mouse, rat and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 25 of Op18 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 25 of Op18 of human origin |
| Entrez Gene | 3925 |
|---|
Application
Antibodies tested include p-Op18 (Ser 25): (A,B) and Op18 (A-4): (C,D).
Application
Western blot analysis of Op18 phosphorylation in untreated (A), PMA treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lysates
Application
Western blot analysis of Op18 (Ser 25) phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lystates.
Application
Western blot analysis of phosphorylated Op18 expression in untreated (A) and PMA-treated (B) Jurkat whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 25 phosphorylated Op18 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
