Application
Western blot analysis of phosphorylated ICAM-1 expression in untreated (A), TNF-α-treated (B) and PMA-treated (C) HUVEC whole cell lysates.| Product Name | p-ICAM-1 Antibody (Tyr 512) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play important, yet separate, roles in embryogenesis and development. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the ∫2å1 and ∫2åM Integrins. ICAM-2 functions as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and is involved in leukocyte adhesion. ICAM-3 is highly expressed on the surface of human eosinophils and, when bound to ligand, may inhibit eosinophil inflammatory responses and survival. ICAM-4, also known as LW glycoprotein, interacts with Integrins åL∫2, åM∫2, å4∫1, the åV family and åIIb∫3, and selective binding to different integrins may be relevant to the pathology in a number of red blood cell associated diseases. Lastly, ICAM-5, expressed on telencephalic neurons, binds CD11a/CD18 and thus may act as an adhesion molecule for leukocyte binding in the central nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 512 phosphorylated ICAM1 of human origin, correspondingly phosphorylated Tyr 518 of mouse origin and correspondingly phosphorylated Tyr 526 of rat origin by WB and IP | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 512 of ICAM-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 512 of ICAM-1 of human origin |
| Entrez Gene | 3383 |
|---|
Application
Western blot analysis of phosphorylated ICAM-1 expression in untreated (A), TNF-α-treated (B) and PMA-treated (C) HUVEC whole cell lysates.| Application Notes | recommended for detection of Tyr 512 phosphorylated ICAM1 of human origin, correspondingly phosphorylated Tyr 518 of mouse origin and correspondingly phosphorylated Tyr 526 of rat origin by WB and IP |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
