Application
Antibodies tested include p-HSP 27 (Ser 82): (A,B,C) and HSP 27 (F-4): (D,E,F).Application
Antibodies tested include p-HSP 27 (Ser 82): (A, B, C) and HSP 27 (M-20): (D, E, F).Application
Western blot analysis of HSP 27 phosphorylation in non-transfected 293T: (A), mouse HSP 27 transfected 293T: (B) and ECV304 (C) whole cell lysates.Application
Western blot analysis of HSP 27 phosphorylation in untreated (A), UV irradiated (B), heat shocked (C), lambda protein phosphatase treated (D), UV irradiated and lambda protein phosphatase treated (E) and heat shocked and lambda protein phosphatase treated (F) HeLa whole cell lysate.Application
Western blot analysis of phosphorylated HSP 27 expression in untreated (A), UV-treated (B) and Heat Shock-treated (C) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | p-HSP 27 Antibody (Ser 82) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 82 phosphorylated HSP 27 of mouse and human origin by WB, IP, IF and IHC(P) | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 82 of HSP 27 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 82 of HSP 27 of human origin |
| Entrez Gene | 3315 |
|---|
Application
Antibodies tested include p-HSP 27 (Ser 82): (A,B,C) and HSP 27 (F-4): (D,E,F).
Application
Antibodies tested include p-HSP 27 (Ser 82): (A, B, C) and HSP 27 (M-20): (D, E, F).
Application
Western blot analysis of HSP 27 phosphorylation in non-transfected 293T: (A), mouse HSP 27 transfected 293T: (B) and ECV304 (C) whole cell lysates.
Application
Western blot analysis of HSP 27 phosphorylation in untreated (A), UV irradiated (B), heat shocked (C), lambda protein phosphatase treated (D), UV irradiated and lambda protein phosphatase treated (E) and heat shocked and lambda protein phosphatase treated (F) HeLa whole cell lysate.
Application
Western blot analysis of phosphorylated HSP 27 expression in untreated (A), UV-treated (B) and Heat Shock-treated (C) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Ser 82 phosphorylated HSP 27 of mouse and human origin by WB, IP, IF and IHC(P) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
