Application
Western blot analysis of RFLAT-1 expression in LADMAC (A), HuT 78 (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of RFLAT-1 expression in K-562 (A) and KNRK (B) whole cell lysates.| Product Name | RFLAT-1 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-180 mapping near the N-terminus of RFLAT-1 of human origin |
| Modification Notes | RANTES factor of late activated T lymphocytes-1 (RFLAT-1), also designated BTEB3 and Kruppel-like factor 13 (KLF13), is a novel transcription factor that is expressed in T cells in the late stages of activation. This delayed induction coincides with the expression of RANTES, a chemoattractant cytokine for monocytes, T lymphocytes, eosinophils, basophils and natural killer cells. RFLAT-1 is localized to the nucleus, where it associates with the A site of the RANTES promoter and, in turn, faciliates transcriptional activation. RFLAT-1 is related to the transcription factor TFIIA-like zinc finger protein superfamily, as it contains three distinct and contigous zinc finger motifs at the carboxy- terminus and a proline-rich transcriptional activation domain, which are also present in TFIIA family of proteins, including Sp1 and Sp3. Although RFLAT-1 activates gene-specific transcription in activated T cells, it is also ubiquitously expressed in various cell types where it is likely regulated by phosphorylation. Late stage transcriptional activation of RANTES in activated T cells is also strongly influenced by Rel proteins of the NF˚B family, suggesting that RFLAT-1 and Rel may synergistically activate the RANTES promoter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RFLAT-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-180 mapping near the N-terminus of RFLAT-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-180 mapping near the N-terminus of RFLAT-1 of human origin |
| Entrez Gene | 51621 |
|---|
Application
Western blot analysis of RFLAT-1 expression in LADMAC (A), HuT 78 (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of RFLAT-1 expression in K-562 (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of RFLAT-1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
