Application
Western blot analysis of IgA1 expression in non-transfected 293T: (A), human IgA1 transfected 293T: (B) and human PBL (C) whole cell lysates.| Product Name | IgA Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of IgA1 of human origin |
| Modification Notes | Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins. The IgA heavy chain is an å-chain, and the light chains are either ˚- or ¬- chains. IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IgA1 and IgA2 of human origin by WB, IP, IF and ELISA: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of IgA1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of IgA1 of human origin |
| Entrez Gene | 3493 |
|---|
Application
Western blot analysis of IgA1 expression in non-transfected 293T: (A), human IgA1 transfected 293T: (B) and human PBL (C) whole cell lysates.| Application Notes | recommended for detection of IgA1 and IgA2 of human origin by WB, IP, IF and ELISA: WB:1:100~1:200 IF:1:20~1:100 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
