Application
Western blot analysis of CYP24 expression in MIA PaCa-2 whole cell lysate (A) and mouse pancreas (B) and mouse liver (C) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.| Product Name | CYP24 Antibody (H-87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 351-437 mapping near the C-terminus of CYP24 of human origin |
| Modification Notes | P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. The P450 family member, CYP19, catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D 25-hydroxylation and is localized to the mitochondria in kidney and liver. Overexpression of CYP24 (encoding vitamin D 24 hydroxylase) is likely to lead to abrogation of growth control mediated by vitamin D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CYP24 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 351-437 mapping near the C-terminus of CYP24 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 351-437 mapping near the C-terminus of CYP24 of human origin |
| Entrez Gene | 1591 |
|---|
Application
Western blot analysis of CYP24 expression in MIA PaCa-2 whole cell lysate (A) and mouse pancreas (B) and mouse liver (C) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.| Application Notes | recommended for detection of CYP24 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
