Application
Western blot analysis of ELP4 expression in NIH/3T3 nuclear extract.Application
Western blot analysis of ELP4 expression in non-transfected: (A) and human ELP4 transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Product Name | ELP4 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In Saccharomyces cerevisiae, the hyperphosphorylated form of RNA polymerase II (RNAP II) mediates transcription elongation, and associates with the elongator complex, which contains six subunits. The elongator complex can be separated into two subcomplexes; one consisting of Elp1, Elp2 and Elp3, and the other consisting of Elp4, Elp5 and Elp6. The elongator complex acetylates both core histones and nucleosomal substrates, and directs its activity specifically towards the N-terminal tails of Histone H3 and Histone H4. An analogous complex exists in mammals and contains a variety of proteins that are functional homologs of their yeast counterparts. ELP4 (elongation protein 4), also known as PAX6NEB, is a 424 amino acid protein that localizes to both the cytoplasm and the nucleus and exists as a component of the elongator complex. Widely expressed as multiple alternatively spliced isoforms, ELP4 is involved in transcriptional regulation and may play a role in chromatin remodeling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ELP4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 48-137 mapping near the N-terminus of ELP4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 48-137 mapping near the N-terminus of ELP4 of human origin |
| Entrez Gene | 26610 |
|---|
Application
Western blot analysis of ELP4 expression in NIH/3T3 nuclear extract.
Application
Western blot analysis of ELP4 expression in non-transfected: (A) and human ELP4 transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).| Application Notes | recommended for detection of ELP4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
