Application
Western blot analysis of UFSP1 expression in non-transfected: (A) and mouse UFSP1 transfected: (B) 293T whole cell lysates.| Product Name | UFSP1 Antibody (H-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4-57 mapping near the N-terminus of UFSP1 of human origin |
| Modification Notes | UFM1 (ubiquitin-fold modifier 1) is a ubiquitin-like protein that is conjugated to target proteins by UBA5, an E1-like activating enzyme, and Ufc1, an E2-like conjugating enzyme. Through these interactions, UFM1 conjugates to target proteins by a covalent linkage. UFSP1 (Ufm1-specific protease 1) is a 142 amino acid thiol protease that cleaves UFM1 precursor and leads to exposure of its conserved C-terminal glycine, a step required prior to conjugation to target proteins. UFSP1 is also capable of releasing UFM1 from UFM1-conjugated cellular proteins. The gene encoding UFSP1 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Defects in genes localized to chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UFSP1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4-57 mapping near the N-terminus of UFSP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4-57 mapping near the N-terminus of UFSP1 of human origin |
| Entrez Gene | 402682 |
|---|
Application
Western blot analysis of UFSP1 expression in non-transfected: (A) and mouse UFSP1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of UFSP1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
