Application
Western blot analysis of PHF5A expression in HeLa whole cell lysate.| Product Name | PHF5A Antibody (V-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | PHF5A (PHD finger protein 5A), also known as SF3b14b or INI, is a 110 amino acid protein that contains one PHD finger-like domain and localizes to the nucleus. One of several members of the PHD finger superfamily, PHF5A functions as a subunit of the SF3A splicing factor complex, a heterotrimeric complex comprised of multiple subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. In conjunction with other members of the SF3A complex, PHF5A plays an important role in mediating pre-mRNA splicing and transcription. The gene encoding PHF5A maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PHF5A of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other PHF family members also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of PHF5A of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of PHF5A of human origin |
| Entrez Gene | 84844 |
|---|
Application
Western blot analysis of PHF5A expression in HeLa whole cell lysate.| Application Notes | recommended for detection of PHF5A of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other PHF family members also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
