Application
Western blot analysis of GITR expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of GITR expression in Dexamethasone treated Hep G2 (A), Jurkat (B), ALL-S1L (C), HuT 78 (D) and H9 (E) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Product Name | GITR Antibody (P-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The tumor necrosis factor receptor (TNFR) superfamily represents a growing family of type I transmembrane glycoproteins that are involved in various cellular functions, including proliferation, differentiation and programmed cell death. These proteins share homology for cysteine-rich repeats in the extracellular ligand binding domain and an intracellular death domain. Members of the TNFR superfamily transmit signals through protein-protein interactions, and these signals can lead to the activation of either the caspase and Jun kinase pathways, which promote cell death, or the NF˚B pathway, which results in cell survival. The glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is a member of the TNFR superfamily that is preferentially expressed in normal T lymphocytes from thymus, spleen and lymph nodes. GITR shares similarity with Ox40, 4-1BB and CD27 and is thought to inhibit T cell receptor-mediated cell death through the activation of the NF˚B signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GITR of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GITR of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 8784 |
|---|
Application
Western blot analysis of GITR expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of GITR expression in Dexamethasone treated Hep G2 (A), Jurkat (B), ALL-S1L (C), HuT 78 (D) and H9 (E) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
