Application
Western blot analysis of MBNL2 expression in SK-N-SH (A) and Caki-1 (B) whole cell lysates.| Product Name | MBNL2 Antibody (Z-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MBNL2 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Pre-mRNA splicing is a critical step in the post-transcriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and MBNL2, associate with expanded CUG repeats in vitro and localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating muscleblind proteins MBNL1 and MBNL2 in the pathogenesis of both disorders. MBNL2, a 367 amino acid protein, participates in recruitment of Intergrin ?3 to focal adhesions in a RNA-dependent protein localization mechanism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MBNL2 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MBNL2 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 10150 |
|---|
Application
Western blot analysis of MBNL2 expression in SK-N-SH (A) and Caki-1 (B) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
