Application
Western blot analysis of Nkx-2.4 expression in mouse testis tissue extract.| Product Name | Nkx-2.4 Antibody (O-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Nkx-2.4 of mouse and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Members of the NK-2 family of homeodomain proteins, which include Nkx-2.2, Nkx-2.4, Nkx-2.5, Nkx-2.6 and many others, are key regulators of growth and development in several tissues, including brain, heart and pancreas. Nkx-2.2 is responsible for directing ventral neuronal patterning in response to graded Shh signaling. Nkx-2.4 is a 354 amino acid nuclear protein that is detected in the posterior hypothalamus of the embryo and also in adult testis. Nkx-2.5, also designated cardiac specific homeobox protein (Csx), is a homolog of the Drosophila tinman protein and is essential for normal cardiovascular development. Nkx-2.6, also a homolog of the Drosophila tinman protein, is expressed in the caudal pharyngeal pouches, the caudal heart progenitors, the sinus venosus, the outflow tract of the heart and in a short segment of the gut between stages E8.5 and E10.5 of embryogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Nkx-2.4 of mouse and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Nkx-2.4 of mouse and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 644524 |
|---|
Application
Western blot analysis of Nkx-2.4 expression in mouse testis tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
