Application
Western blot analysis of NNP-1 expression in non-transfected: (A) and human NNP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NNP-1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Product Name | NNP-1 Antibody (P-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NNP-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Novel nuclear protein 1 (NNP-1), also known as RRP1-like protein or Nucleolar protein Nop52, is a 461 amino acid protein belonging to the RRP1 family. Localized to the nucleolus, NNP-1 has simian virus 40-type and bipartite nuclear localization signals and four coiled-coil domains within its C-terminal region. NNP-1 has been found to play an important role in the generation of 28S rRNA in the late processing steps of ribosome biogenesis. At the end of mitosis, nucleolar proteins assemble in a sequential order during the rebuilding of the nucleolus. NNP-1 assembles after Fibrillarin and C23, and simultaneously with B23 and POP1 in the prenucleolar body pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NNP-1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NNP-1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 8568 |
|---|
Application
Western blot analysis of NNP-1 expression in non-transfected: (A) and human NNP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NNP-1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
