Application
Western blot analysis of CITED4 expression in SP2/0 whole cell lysate.| Product Name | CITED4 Antibody (A-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CITED4 of mouse and rat origin by WB, IP and ELISA |
| Modification Notes | CITED4 (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4), also designated transcriptional coactivator 4, interacts via its C-terminus with the cysteine-histidine-rich domain 1 (CH1) of EP300 (p300) and CREB-binding protein (CBP). CITED4 may inhibit interaction of hypoxia-inducible factor 1 å (HIF-1å) with CBP, thus inhibiting transactivation by HIF1A. CITED4 also interacts as a transcriptional coactivator with all isoforms of TFAP2. Nuclear loss or cytoplasmic translocation of CITED4, followed by a loss of HIF-1å transcriptional antagonist activity, indicates breast cancer development. CITED4 expression occurs in a variety of tissues including heart, liver, skeletal muscle, pancreas and various breast cancer cell lines. CITED4 is highly expressed in embryonic endothelial cells and mammary epithelial cells of pregnant and lactating females, respectively. Regulation of CITED4 may occur through the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CITED4 of mouse and rat origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CITED4 of mouse and rat origin by WB, IP and ELISA |
| Entrez Gene | 56222 |
|---|
Application
Western blot analysis of CITED4 expression in SP2/0 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
