Application
Western blot analysis of TTLL1 expression in non-transfected: (A) and human TTLL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TTLL1 expression in mouse heart tissue extract.| Product Name | TTLL1 Antibody (T-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of TTLL1 of human origin |
| Modification Notes | A large protein group known as the tubulin tyrosine ligase-like family (TTLL) family is implied to catalyze ligations of amino acids to tubulins and other substrates. Each member contains a characteristic TTL domain. TTLL1 (tubulin tyrosine ligase-like family, member 1), also known as tubulin polyglutamylase complex subunit 3, PGs3 or C22orf7, is a 423 amino acid catalytic subunit of the neuronal tubulin polyglutamylase complex and a member of the tubulin polyglutamylase family. Localized to cytoskeleton and cytosol, TTLL1 is widely expressed with highest levels found in brain, testis and heart. TTLL1 generates glutamate side chains on C-terminal regions of å- and ∫-Tubulin and contains one TTL domain. Four TTLL1 isoforms are known to exist as a result of alternative splicing events. The gene encoding TTLL1 maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TTLL1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with isoform TTLL1b also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of TTLL1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of TTLL1 of human origin |
| Entrez Gene | 25809 |
|---|
Application
Western blot analysis of TTLL1 expression in non-transfected: (A) and human TTLL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TTLL1 expression in mouse heart tissue extract.| Application Notes | recommended for detection of TTLL1 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with isoform TTLL1b also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
