Application
Western blot analysis of PSMD11 expression in human fetal brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Product Name | PSMD11 Antibody (O-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PSMD11 of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | In eukaryotic cells, the selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S proteasome. The 26S proteasome is a protease complex that selectively breaks down proteins that have been modified by polyubiquitin chains. It is made up of two multisubunit complexes: the 20S proteasome chamber, which serves as the proteolytic core of the complex, and two 19S regulatory particles, which recognize and unfold ubiquitinated proteins. PSMD11 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 11), also known as S9, Rpn6 or p44.5, is a 422 amino acid protein that contains one PCI domain and functions as a regulatory subunit of the 26S proteasome, playing a role in the ATP-dependent degradation of ubiquitinated proteins. The gene encoding PSMD11 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PSMD11 of mouse rat human and zebrafish origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of PSMD11 of mouse, rat, human and zebrafish origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 5717 |
|---|
Application
Western blot analysis of PSMD11 expression in human fetal brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
