Application
Western blot analysis of SF4 expression in Hep G2 whole cell lysate.| Product Name | SF4 Antibody (B-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SF4 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | SF4 (splicing factor 4), also known as RNA-binding protein (RBP) or F23858, is a 645 amino acid nuclear protein and member of the SURP family of splicing factors that is involved in pre-mRNA splicing. Existing as two alternatively spliced isoforms and highly expressed in testis and heart, SF4 is also found in skeletal muscle, brain and kidney where it functions as a component of the spliceosome. SF4 contains two SURP motif repeats, one G-patch domain and is encoded by a gene that maps to human chromosome 19p13.11. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcRs). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SF4 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SF4 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 57794 |
|---|
Application
Western blot analysis of SF4 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
