Application
Western blot analysis of RBMX expression in non-transfected: (A) and mouse RBMX transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RBMX expression in K-562 nuclear extract.| Product Name | RBMX Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 312-391 mapping at the C-terminus of RBMX of human origin |
| Modification Notes | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome . The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. RBMX (also known as hnRNP G) is a glycoprotein originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding RBMX is located on human chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and RBMXL2, which is a testis specific protein. All three proteins interact with Tra2b, and therefore are involved in pre-mRNA splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of RBMX of human, mouse, and rat origin, and RBMXRT of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 312-391 mapping at the C-terminus of RBMX of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 312-391 mapping at the C-terminus of RBMX of human origin |
| Entrez Gene | 27316 |
|---|
Application
Western blot analysis of RBMX expression in non-transfected: (A) and mouse RBMX transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RBMX expression in K-562 nuclear extract.| Application Notes | recommended for detection of RBMX of human, mouse, and rat origin, and RBMXRT of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
