Application
Western blot analysis of C-TAK1 expression in non-transfected: (A) and human C-TAK1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C-TAK1 expression in non-transfected: (A) and human C-TAK1 transfected: (B) 293T whole cell lysates.| Product Name | MARK3 Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 447-501 mapping within an internal region of MARK3 of human origin |
| Modification Notes | C-TAK1 (Cdc25C associated protein kinase) phosphorylates Cdc25C on Ser 216 and is ubiquitously expressed in various human tissue and cell lines. C-TAK1 is distinct from Chk1, which also phosphorylates Cdc25C on Ser 216 in response to DNA damage. Phosphorylation of Cdc25C allows for the preferential binding of 14-3-3 proteins, subsequently retaining Cdc25C in the cytoplasm. Thus, the binding of 14-3-3 proteins prevents Cdc25C from dephosphorylating Cdc2 in the nucleus, thereby controlling the entry of the cells into mitosis. It is suggested that C-TAK1 mediates the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MARK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 447-501 mapping within an internal region of MARK3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 447-501 mapping within an internal region of MARK3 of human origin |
| Entrez Gene | 4140 |
|---|
Application
Western blot analysis of C-TAK1 expression in non-transfected: (A) and human C-TAK1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C-TAK1 expression in non-transfected: (A) and human C-TAK1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MARK3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
