Application
Western blot analysis of palladin expression in 3611-RF (A) and CCD-1064Sk (B) whole cell lysates.| Product Name | palladin Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 361-460 mapping within an internal region of palladin of human origin |
| Modification Notes | Palladin, also known as PALLD, PNCA1 or SIH002, is a 1,383 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains five immunoglobulin (Ig)-like domains. Expressed as several alternatively spliced isoforms that are found in kidney, prostate, ovary and colon, palladin functions as a cytoskeletal protein that is required both for the organization of the Actin cytoskeleton, as well as for the establishment of proper cell motility, cell adhesion and cell-matrix interactions. Palladin interacts with Eps8, LASP-1 and VASP and may also play a role in cytoskeletal scaffolding and Actin remodeling. In response to DNA damage, palladin is subject to phosphorylation on select serine residues. Defects in the gene encoding palladin may increase genetic susceptibility to pancreatic cancer, strongly suggesting a role for palladin in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of palladin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 361-460 mapping within an internal region of palladin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 361-460 mapping within an internal region of palladin of human origin |
| Entrez Gene | 23022 |
|---|
Application
Western blot analysis of palladin expression in 3611-RF (A) and CCD-1064Sk (B) whole cell lysates.| Application Notes | recommended for detection of palladin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
