Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of CacyBP expression in Hep G2 whole cell lysate.Application
Western blot analysis of CacyBP expression in WI 38 whole cell lysate (A) and mouse embryo tissue extract (B).Application
Western blot analysis of CacyBP expression in WI 38 whole cell lysate.| Product Name | CacyBP Antibody (FL-228) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-228 representing full length CacyBP of human origin |
| Modification Notes | CacyBP (Calcyclin-binding protein, Siah-interacting protein) is a 228 amino acid protein encoded by the human gene CACYBP. CacyBP is primarily a nuclear protein that contains one CS domain and one SGS domain. CacyBP is believed to be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It most likely serves as a molecular bridge in ubiquitin E3 complexes. It also participates in the ubiquitin-mediated degradation of b-catenin. CacyBP is thought to be a potential inhibitor of cell growth and invasion in the gastric cancer cell through its effects on b-catenin protein expression and transcriptional activation of TCF/LEF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CacyBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-228 representing full length CacyBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-228 representing full length CacyBP of human origin |
| Entrez Gene | 27101 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of CacyBP expression in Hep G2 whole cell lysate.
Application
Western blot analysis of CacyBP expression in WI 38 whole cell lysate (A) and mouse embryo tissue extract (B).
Application
Western blot analysis of CacyBP expression in WI 38 whole cell lysate.| Application Notes | recommended for detection of CacyBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
