Application
Western blot analysis of IARS2 expression in non-transfected: (A) and human IARS2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.| Product Name | IARS2 Antibody (T-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of IARS2 of human origin |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Proteins belonging to this family function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. IARS2 (Isoleucyl-tRNA synthetase), also known as IleRS (Isoleucine-tRNA ligase), is a 1012 amino acid protein that is localized within the mitochondrial matrix. Specifically belonging to the class I mitochondrial aminoacyl-tRNA synthetase family, IARS2 contains a characteristic Rossman fold, which is found in proteins that typically bind nucleotides. The gene encoding IARS2 maps to human chromosome one, which is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IARS2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of IARS2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of IARS2 of human origin |
| Entrez Gene | 55699 |
|---|
Application
Western blot analysis of IARS2 expression in non-transfected: (A) and human IARS2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.
Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.| Application Notes | recommended for detection of IARS2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
