Application
Western blot analysis of PAT2 expression in 293 whole cell lysate.| Product Name | PAT2 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PAT2 of human origin by WB, IP and ELISA |
| Modification Notes | The proton-coupled amino acid transporter family consists of four family members, namely PAT1, PAT2, PAT3 and PAT4, all of which mediate the 1:1 symport of protons and small neutral amino acids and derivatives across both intracellular and plasma membranes. Substrates for the PAT family members include L- and D-proline, glycine and L-alanine, 3-amino-1-propanesulfonic acid, L-azetidine-2-carboxylic acid and cis-4-hydroxy-D-proline. PAT1 expression is high in intestine and brain where it localizes to the brush border membrane, thereby allowing PAT1 to serve as a novel route for oral drug delivery. PAT2 shows high expression in spinal cord and brain, while PAT3 expression is found in testis. PAT4 is a multi-pass membrane protein that is expressed as two alternatively spliced isoforms. All four PAT family members contain three conserved histidine residues with His-55 found to be essential for catalytic activity of PAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:100~1:200 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PAT2 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PAT2 of human origin by WB, IP and ELISA |
| Entrez Gene | 153201 |
|---|
Application
Western blot analysis of PAT2 expression in 293 whole cell lysate.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
