Application
Western blot analysis of ZNF274 expression in Jurkat whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear and cytoplasmic staining.| Product Name | ZNF274 Antibody (E-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against ZNF274 of human origin |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. A member of the Krüppel C2H2-type zinc-finger protein family, ZNF274, also known as Zinc finger protein with KRAB and SCAN domains 19, is a 653 amino acid protein containing five C2H2-type zinc fingers, two KRAB A domains and one SCAN box domain. Predominantly localized to the nucleolus, ZNF274 exhibits transcriptional repressing activity. There are four isoforms of ZNF274 that are produced as a result of alternative splicing events. Although total ZNF274 expression seems to be ubiquitous, the two main isoforms, ZNF274a and ZNF274b, differ slightly in tissue distribution with higher expression of ZNF274a in testis and higher expression of ZNF274b in spleen, ovary, skeletal muscle and thymus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ZNF274 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against ZNF274 of human origin |
|---|---|
| Tissue Specificity | raised against ZNF274 of human origin |
| Entrez Gene | 10782 |
|---|
Application
Western blot analysis of ZNF274 expression in Jurkat whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human heart tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of ZNF274 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
