Application
Western blot analysis of CCDC16 expression in non-transfected 293T: (A), human CCDC16 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CCDC16 expression in Jurkat whole cell lysate.| Product Name | CCDC16 Antibody (O-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against CCDC16 of human origin |
| Modification Notes | CCDC16 (coiled-coil domain-containing protein 16), also known as ZNF830, OMCG1 (ovum mutant candidate gene 1) or SEL13, is a 372 amino acid protein that belongs to the C2H2-type zinc finger family of proteins. Localizing to the nucleus, CCDC16 contains one U1-type zinc finger motif and is involved in pre-mRNA splicing. CCDC16 functions as a component of a pre-mRNA splicing complex of the spliceosome (composed of AQR (aquarius), PRP19, CCDC16, HCNP, ISY1 and Cyclophilin E) and is required for proper RNA synthesis in the cell. Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. In preimplantation embryos, CCDC16 is believed to play an important role in cell cycle regulation. Further supporting its vital role in embryos, the disruption of CCDC16 leads to early embryonic lethality. Upon DNA damage, CCDC16 is phosphorylated by ATM or ATR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of CCDC16 of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against CCDC16 of human origin |
|---|---|
| Tissue Specificity | raised against CCDC16 of human origin |
| Entrez Gene | 91603 |
|---|
Application
Western blot analysis of CCDC16 expression in non-transfected 293T: (A), human CCDC16 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CCDC16 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CCDC16 of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
