Application
Western blot analysis of Dvl-3 expression in non-transfected: (A) and human Dvl-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Dvl-2 expression in non-transfected: (A) and human Dvl-2 transfected: (B) 293 whole cell lysates.| Product Name | Dvl Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-280 mapping near the N-terminus of Dvl-1 of human origin |
| Modification Notes | Mammalian homologs of the Drosophila dishevelled (Dsh) gene have been identified, including Dvl-1, Dvl-2 and Dvl-3 (1-5). The mammalian dishevelled proteins contain three homologous domains, two of which are unrelated to any other known protein. The third region is homologous to the discs-large homology domain of Drosophila discs-large-1, a tumor suppressor protein (1,2). Like their Drosophila counterpart, the dishevelled proteins are thought to be involved in embryogenesis (5). Overexpression of Dvl-1 has been shown to inhibit the phosphorylation of tau by GSK-3ß. This finding may prove to be important in Alzheimer’s studies, which have shown that tau is hyperphosphorylated (6). In Drosophila, Dsh is a component of the frizzled signaling pathway (7). Both mammalian dishevelled and frizzled proteins are components of the Wnt signalling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dvl-1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-280 mapping near the N-terminus of Dvl-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-280 mapping near the N-terminus of Dvl-1 of human origin |
| Entrez Gene | 1855 |
|---|
Application
Western blot analysis of Dvl-3 expression in non-transfected: (A) and human Dvl-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Dvl-2 expression in non-transfected: (A) and human Dvl-2 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of Dvl-1, 2 and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
