Application
Western blot analysis of MAPKAPK-2 phosphorylation in untreated (A), UV irradiated (B) and UV irradiated and lambda protein phosphatase treated (C) HeLa whole cell lysates.Application
Western blot analysis of MAPKAPK-2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Blots were probed with p-MAPKAPK-2 (Thr 334): preincubated with its cognate phosphorylated peptide (A) and p-MAPKAPK-2 (Thr 334): (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Product Name | p-MAPKAPK-2 Antibody (Thr 334) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 334 of MAPKAPK-2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The p38 mitogen-activated protein kinase (MAPK) pathway is an important mediator of cellular responses to environmental stress. The MAPKAP kinases (MAP kinase activated protein kinases) are a group of MAP kinase substrates that are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The activated MAPKAPK2 phosphorylates its nuclear targets CREB/ATF1, serum response factor, and E2A protein E47 and its cytoplasmic targets HSP25/27, LSP-1, 5-lipoxygenase, glycogen synthase, and tyrosine hydroxylase. Phosphorylation of threonine 334, which is located between the kinase domain and the C-terminal regulatory domain, may serve as a switch for MAPKAPK2 nuclear import and export. Threonine 222 which lies in the activation loop is also phosphorylated. Phosphorylated MAPKAPK2 masks the nuclear localization signal at its C terminus by binding to p38, and unmasks the nuclear export signal, carrying p38 to the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 334 phosphorylated MAPKAPK-2 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 334 of MAPKAPK-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 334 of MAPKAPK-2 of human origin |
| Entrez Gene | 9261 |
|---|
Application
Western blot analysis of MAPKAPK-2 phosphorylation in untreated (A), UV irradiated (B) and UV irradiated and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Application
Western blot analysis of MAPKAPK-2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Blots were probed with p-MAPKAPK-2 (Thr 334): preincubated with its cognate phosphorylated peptide (A) and p-MAPKAPK-2 (Thr 334): (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Application Notes | recommended for detection of Thr 334 phosphorylated MAPKAPK-2 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
