Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.Application
Western blot analysis of CIN85 expression in non-transfected: (A) and human CIN85 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CIN85 expression in non-transfected 293T: (A), human CIN85 transfected 293T: (B), THP-1 (C) and RAW 264.7 (D) whole cell lysates.Application
Western blot analysis of CIN85 expression in Jurkat (A) and THP-1 (B) whole cell lysate.| Product Name | CIN85 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 366-665 mapping at the C-terminus of CIN85 of human origin |
| Modification Notes | CD2AP (CMS) and CIN85 (Ruk) belong to a family of ubiquitously expressed adaptor molecules containing three SH3 domains, a proline-rich region and a coiled-coil domain. By binding to numerous proteins, CD2AP and CIN85 assemble multimeric complexes implicated in cell-specific signals controlling T-cell activation, kidney glomeruli function or apoptosis in neuronal cells. CIN85/CD2AP also associate with accessory endocytic proteins, components of the actin cytoskeleton, and other adaptor proteins involved in receptor tyrosine kinase signaling. These interactions enable CIN85/CD2AP to function within a network of signaling pathways that coordinate critical steps involved in downregulation and degradation of receptor tyrosine kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CIN85 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 366-665 mapping at the C-terminus of CIN85 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 366-665 mapping at the C-terminus of CIN85 of human origin |
| Entrez Gene | 30011 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in germinal and non-germinal centers.
Application
Western blot analysis of CIN85 expression in non-transfected: (A) and human CIN85 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CIN85 expression in non-transfected 293T: (A), human CIN85 transfected 293T: (B), THP-1 (C) and RAW 264.7 (D) whole cell lysates.
Application
Western blot analysis of CIN85 expression in Jurkat (A) and THP-1 (B) whole cell lysate.| Application Notes | recommended for detection of CIN85 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
