Application
Immunofluorescence staining of methanol-fixed A-431 cells treated with Pervanadate (Sodium Orthovanadate: + Hydrogen Peroxide: ) (A) untreated (B) and treated with Pervanadate and Lambda Phosphatase: (C).Application
Antibodies tested include p-caveolin-1 (Tyr 14): (A,B,C) and caveolin-1 (N-20): (D,E,F).Application
Western blot analysis of phosphorylated caveolin-1 expression in untreated (A) and H2O2-treated (B) NIH/3T3 whole cell lysates.| Product Name | p-caveolin-1 Antibody (Tyr 14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 14 of caveolin-1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated å and ∫, share a distinct but overlapping cellular distribution and differ by an amino-terminal 31 amino acid sequence which is absent from the b isoform. caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms. Caveolin-1 is presumed to be phosphorylated by c-Src kinase, although little is known about this phosphorylation event. Tyrosine 14 of caveolin-1 undergoes regulated phosphoryl-ation during growth factor signaling and is constitutively phosphorylated in Src- and Abl-transformed cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| recommended for detection of Tyr 14 phosphorylated caveolin-1 of mouse, rat and human origin by WB, IP and IF: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 14 of caveolin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 14 of caveolin-1 of human origin |
| Entrez Gene | 857 |
|---|
Application
Immunofluorescence staining of methanol-fixed A-431 cells treated with Pervanadate (Sodium Orthovanadate: + Hydrogen Peroxide: ) (A) untreated (B) and treated with Pervanadate and Lambda Phosphatase: (C).
Application
Antibodies tested include p-caveolin-1 (Tyr 14): (A,B,C) and caveolin-1 (N-20): (D,E,F).
Application
Western blot analysis of phosphorylated caveolin-1 expression in untreated (A) and H2O2-treated (B) NIH/3T3 whole cell lysates.| Application Notes | recommended for detection of Tyr 14 phosphorylated caveolin-1 of mouse, rat and human origin by WB, IP and IF: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
