Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.Application
Antibodies tested include p-Bcl-2 (74.Ser 70): (A,B) and Bcl-2 (C-2): (C,D).Application
Western blot analysis of Bcl-2 phosphorylation in untreated (A), paclitaxel treated (B) and paclitaxel and lambda protein phosphatase treated (C) Jurkat whole cell lysates.| Product Name | p-Bcl-2 Antibody (74.Ser 70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a short amino acid sequence containing phosphorylated Ser 70 of Bcl-2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Apoptosis is defined as a set of cascades which, when initiated, programs the cell to undergo lethal changes such as membrane blebbing, mitochondrial break down and DNA fragmentation. Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an anti-apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xl, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the anti-apoptotic influence of Bcl-2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Rabbit |
| Tested Applications | WBIPIHCELISA |
| recommended for detection of Ser 70 phosphorylated Bcl-2 of human origin by WB, IP, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a short amino acid sequence containing phosphorylated Ser 70 of Bcl-2 of human origin |
|---|---|
| Tissue Specificity | raised against a short amino acid sequence containing phosphorylated Ser 70 of Bcl-2 of human origin |
| Entrez Gene | 596 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.
Application
Antibodies tested include p-Bcl-2 (74.Ser 70): (A,B) and Bcl-2 (C-2): (C,D).
Application
Western blot analysis of Bcl-2 phosphorylation in untreated (A), paclitaxel treated (B) and paclitaxel and lambda protein phosphatase treated (C) Jurkat whole cell lysates.| Application Notes | recommended for detection of Ser 70 phosphorylated Bcl-2 of human origin by WB, IP, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
