Application
Western blot analysis of MFG-E8 expression in MCF7 whole cell lysate.| Product Name | MFG-E8 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 325-384 mapping near the C-terminus of MFG-E8 of human origin |
| Modification Notes | Human milk-fat globule (MFG) is abundant in human breast milk and is composed of secreted lipids encapsulated by plasma membranes from the epithelial cells of mammary glands. MFG membranes are composed of various glycoproteins that serve as markers for differentiated carcinomas. MFG-E8 (Milk fat globule-EGF factor 8), also known as Lactadherin or BA46, is a 387 amino acid peripheral membrane protein that localizes to the membrane of a variety of tissues, including mammary epithelial surfaces, and contains one EGF-like domain and two F5/8 type C domains. Functioning as a specific ligand for Integrin ∫5 and Integrin ∫3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MFG-E8 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 325-384 mapping near the C-terminus of MFG-E8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 325-384 mapping near the C-terminus of MFG-E8 of human origin |
| Entrez Gene | 4240 |
|---|
Application
Western blot analysis of MFG-E8 expression in MCF7 whole cell lysate.| Application Notes | recommended for detection of MFG-E8 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
