Application
Western blot analysis of TRPV5 expression in mouse kidney tissue extract.Application
Western blot analysis of TRPV5 expression in mouse liver tissue extract.Application
Western blot analysis of TRPV5 expression in Caki-1 (A), Hep G2 (B), H4 (C), MG-63 (D) and HOS (E) whole cell lysates.| Product Name | TRPV5 Antibody (H-99) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 631-729 mapping at the C-terminus of TRPV5 of human origin |
| Modification Notes | Transient receptor potential (TRP) proteins are cation-sensitive channels that modulate a myriad of cellular functions, including temperature sensation and vasoregulation Transcribed from a gene adjacent to VR-1, the thermal-sensitive, capsaicin-insensitive TRPV3 is expressed at warm temperatures; expression increases in response to noxious temperatures. Human TRPV3 is expressed in skin, tongue, dorsal root ganglion, trigeminal ganglion, spinal cord and brain. In addition, TRPV3 is co-expressed in dosal root ganglion neurons with VR-1. TRPV3 associates with VR-1 and may modulate VR-1 activity. The 729 amino acid TRPV5 (ECAC1) protein comprises six transmembrane domains, multiple potential phosphorylation sites, an N-linked glycosylation site and three ankyrin repeat regions. It is abundantly expressed in kidney, jejunum and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. TRPV5 controls the rate-limiting step of vitamin D3-regulated Ca2+ reabsorption in kidney and intestine; the 5’-flanking region of TRPV5 contains four putative vitamin D3-responsive elements. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRPV5 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 631-729 mapping at the C-terminus of TRPV5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 631-729 mapping at the C-terminus of TRPV5 of human origin |
| Entrez Gene | 56302 |
|---|
Application
Western blot analysis of TRPV5 expression in mouse kidney tissue extract.
Application
Western blot analysis of TRPV5 expression in mouse liver tissue extract.
Application
Western blot analysis of TRPV5 expression in Caki-1 (A), Hep G2 (B), H4 (C), MG-63 (D) and HOS (E) whole cell lysates.| Application Notes | recommended for detection of TRPV5 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
