Application
Western blot analysis of Proteassemblin expression in HL-60 (A), HeLa (B) and SW480 (C) whole cell lysates.| Product Name | Proteassemblin Antibody (FL-141) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-141 representing full length Proteassemblin of human origin |
| Modification Notes | Proteassemblin, also known as POMP (proteasome maturation protein), UMP1 or Voltage-gated potassium channel ∫ subunit 4.1, is an endoplasmic reticulum (ER) associated protein that functions as a molecular chaperone required for proteasome and immunoproteasome assembly. Essential for cell viability and induced by IFN-©, Proteassemblin associates with preproteasomes and specifically binds to Proteasome 20S ∫1i, ∫1, ∫5, ∫6 and ∫7 subunits. Proteassemblin is responsible for mediating the binding of the 20S preproteasome to the ER membrane and is required for incorporation of the ∫ subunits into the 20S proteasome. Proteassemblin is the human homolog of the yeast Ump1 protein. Unlike Ump1, which becomes incorporated into the proteasome, Proteassemblin is degraded upon maturation of the newly formed proteasome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Proteassemblin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-141 representing full length Proteassemblin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-141 representing full length Proteassemblin of human origin |
| Entrez Gene | 51371 |
|---|
Application
Western blot analysis of Proteassemblin expression in HL-60 (A), HeLa (B) and SW480 (C) whole cell lysates.| Application Notes | recommended for detection of Proteassemblin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
